加兰是国际杀手组织的一员，他们与世界上最危险的杀手签约，但却发现他们是被追捕的人。

在《复仇者联盟：终局之战》之后，萨姆·威尔逊（猎鹰）和巴奇·巴恩斯（冬兵）联合参与了一场全球性冒险行动，他们的能力和耐心受到考验。

His passion for combat sports wasn't keeping him alive so survival mode kicked in for Michael and he was forced to take normal jobs, including working in factories, slaughterhouses, demolition companies; in addition to working as an upholsterer, postman, tiler, plasterer, and salesman. In his later years though, Michael's career as a UFC fighter would take off and he would become one of the sports fan favorites, but with any combat sport comes the shrapnel of getting kicked and punched in the face on a nightly basis. In a controversial fight against the steroid-induced Victor Belfort, the unthinkable would happen to change the course of Bisping's life forever. He would suffer a detached retina from a blow and kick to the head that would disfigure and scar the fighter forever, threatening to end a career he has sacrificed everything for. With a wife and kids that love him, they plead for the battered and bruised fighter to hang up the gloves, as they feel he has nothing left to prove. But in the end that decision is left to only one man: Michael Bisping.

渐渐习惯了现代生活的美国队长史蒂夫·罗杰斯（克里斯·埃文斯 Chris Evans 饰），在一次行动后隐隐嗅到神盾局内部所弥漫出来的凶险气味。而当得知神盾局正秘密进行的“洞察计划”后，他更为此感到愤怒。某天，神盾局指挥官尼克-法瑞（塞缪尔·杰克逊 Samuel L. Jackson 饰）遭到一群武装分子袭击，他拼尽全力将一支U盘交到美国队长手中，这里面藏着该局二战以来最重要且最可怕的秘密。在此之后，美国队长因涉嫌杀害尼克而遭到前局长亚历山大·皮尔斯（罗伯特·雷德福/Robert Redford 饰）的通缉和追杀，逃亡中他得到黑寡妇（斯嘉丽·约翰逊 Scarlett Johansson 饰）和退伍老兵“猎鹰”（安东尼·麦凯 Anthony Mackie 饰）的帮助。
　　他们必须争分夺秒解开神盾局的秘密，阻止“洞察计划”的施行……